TRID :
6056135
Actual Tender Number :
AOC
Supply of consumables, ssoadvanced universal sybr green supermix (
1725271) biorad, 2 iscript cdna synthesis kit (
1708891) biorad, 3 10% tween 20 solution, (
1662404), 5 ml biorad, 4 30% acrylamide/bis solution, 29:1 (
1610156), 500 ml biorad, 5 ammonium persulfate (aps) (
1610700), 10 g biorad, 6 temed (
1610800), 5 ml biorad, 7 precision plus protein ladder, prestained, all blue (
1610373), 50 app 2 biorad, 8 16-tube magnetic rack (
1614916) biorad, 9 immun-blot low fluorescence pvdf/filter paper sets (
1620260) biorad, 10 proflow sort grade 8x sheath fluid (
12012932) 4 biorad, 11 proline™ rainbow beads (
1451085) 2 biorad, 12 proline universal calibration beads (
1451086) 4 biorad, 13 s3e nozzle tip, 100 µm (
1441009) biorad, 14 2x laemmli buffer (
1610737edu), 30 ml biorad, 15 serological pipette, sterile, individually packed, 5 ml, 200/case vwr or nunc, 16 serological pipette, sterile, individually packed, 10 ml, 200/case vwr or nunc, 17 35 mm cell culture dish, tc surface, 400-500/case vwr or nunc, 18 60 mm cell culture dish, tc surface, 200-250/case vwr or nunc, 19 100 mm cell culture dish, tc surface, 100-150/case vwr or nunc, 20 t25 flask, tc surface, filter cap, 10/pack vwr or nunc, 21 6-well plate, tc surface, 50-100/pack vwr or nunc, 22 24-well plate, tc surface, 50-100/pack vwr or nunc, 23 96-well plate, tc surface, 50-100/pack vwr or nunc, 24 western blot box, clear, 7.3× 5.1× 3.2 cm, reusable, 5/pack sigma, 25 dmso for cell culture, hybri-max (
d2650-5x5ml) sigma, 26 dialysis tubing closures, 50 mm, 1 pack (pack of 10) (
z371130-10ea) sigma, 27 dialysis tubulin, 32 mm width, mwco 12.4 (
d0530) sigma, 28 anti-lamin b antibody, nuclear envelop marker, 20 ul (
10895-1-ap) proteintech, 29 anti-gm 130 antibody as golgi marker, 20 ul (
11308-1-ap) proteintech, 30 anti-calreticulin antibody as er marker, 20 ul (
27298-1-ap) proteintech, 31 anti-cytokeratin antibody-if marker, 20 ul (
22208-1-ap) proteintech, 32 anti- alpha tubulin monoclonal antibody, 20 ul (
66031-1-ig) 1 proteintech, 33 slowfade gold antifade mountant, 2 ml; (
s36940) 2 thermo, 34 purelink quick plasmid miniprep kit (
k210010), 250 prep 1 thermo, 35 hispur ni-nta superflow agarose (
25214), 10 ml 1 thermo, 36 purelink quick gel extraction and pcr purification kit (
k220001) 1 thermo, 37 lipofectamine 3000 transfection reagent, 0.75 ml (
l3000008) 1 thermo, 38 trizol reagent (
15596026) 1 thermo, 9 | p a g e, 39 fbs (south american origin, 50 ml), gibco (
a3160801) 1 thermo, 40 fbs, certified, usa, 50 ml, gibco (
a3160401) 1 thermo, 41 penicillin-streptomycin (10, 000 u/ml)(
15140122), 100 ml 1 thermo, 42 gibco dpbs for cell culture, 500 ml (
14190144) 2 thermo, 43 pierce 16% formaldehyde, methanol-free (
28908) 1 thermo, 44 pierce protein a/g magnetic agarose beads (
78609), 1 ml 1 thermo, 45 restriction enzyme bamhi-hf, 20000 u/ml, 10000 u (
r3136s) 1 neb, 46 restriction enzyme ecori-hf, 20000 u/ml, 10000 u (
r3101s) 1 neb, 47 restriction enzyme hindiii-hf, 20000 u/ml, 10000 u (
r3104s) 1 neb, 48 agar agar, type i (
grm666), 500 g 1 himedia, 49 acetosyringon, (
pct1301), 5g 1 himedia, 50 nutrient broth (
m002), 500 g 1 himedia, 51 luria bertani broth, miller ph 7.0 (
m2119), 500 g 1 himedia, 52 bacterial genomic dna purification kit (
mb505-50pr) 1 himedia, 53 agarose special, low eeo, 100 g (
rm273) 1 himedia, 54 agarose, low melting, 20-25 g (
rm861) 1 himedia, 55 dmem high glucose with 4.5g glucose/l, sodium bicarbonate and, sodium pyruvate w/o l-glutamine (
al007), 500 ml, 1 himedia, 56 l-glutamine 200mm solution l-glutamine in 0.85% normal saline cell, culture tested (
tcl012), 1 himedia, 57 hiper sandwich elisa teaching kit (
hti014) 1 himedia, 58 hiper dot elisa teaching kit product (
hti015) 1 himedia, 59 hiper ouchterlony double diffusion teaching kit (
hti003) 1 himedia, 60 hiper radial immunodiffusion kit (
hti004) 1 himedia, 61 hiper igg purification teaching kit (hti022) 1 himedia, 62 0.22 micron syringe filter, pre-sterile, pes membrane, 50-60/box 1 pall, 63 phalloidin-ifluor 647 reagent (
ab176759) 1 abcam, 64 comet assay slide (3-well) (
ab238546), 5 slides 1 abcam, 65 mouse il-12 p70 elisa kit (
ab119531) 1 abcam, 66, annexin v-fitc apoptosis staining / detection kit (
ab14085), 1 abcam/bd, bioscience, 67, propidium iodide (ab14083), 1 abcam/bd, bioscience, 68 bd multitest anti-human cd45ra fitc/cd45ro pe/cd3, percp/cd4 apc; size: 50 tests (
340571), 1 bd bioscience, 69 bd pharmingen alexa fluor 488 hamster anti-mouse cd3e clone 145-, 2c11 (
557666), size: 0.1 mg, 1 bd bioscience, 70 bd pharmingen percp-cy5.5 rat anti-mouse cd19 clone 1d3, (
561113), size: 25 ug, . at Kolkata,West Bengal,India